U.S. Department of Energy

Pacific Northwest National Laboratory

Multi-platform 'Omics Analysis of Human Ebola Virus Disease Pathogenesis

TitleMulti-platform 'Omics Analysis of Human Ebola Virus Disease Pathogenesis
Publication TypeJournal Article
Year of Publication2017
AuthorsEisfeld A.J, Halfmann P.J, Wendler J.P, Kyle J.E, Burnum-Johnson K.E, Peralta Z., Maemura T., Walters K.B, Watanabe T., Fukuyama S., Yamashita M., Jacobs J.M, Kim Y.M, Casey C.P, Stratton K.G, Webb-Robertson B.M, Gritsenko M.A, Monroe M.E, Weitz K.K, Shukla A.K, Tian M., Neumann G., Reed J.L, van Bakel H., Metz T.O, Smith R.D, Waters K.M, N'jai A., Sahr F., Kawaoka Y.
JournalCell Host Microbe
Abstract

The pathogenesis of human Ebola virus disease (EVD) is complex. EVD is characterized by high levels of virus replication and dissemination, dysregulated immune responses, extensive virus- and host-mediated tissue damage, and disordered coagulation. To clarify how host responses contribute to EVD pathophysiology, we performed multi-platform 'omics analysis of peripheral blood mononuclear cells and plasma from EVD patients. Our results indicate that EVD molecular signatures overlap with those of sepsis, imply that pancreatic enzymes contribute to tissue damage in fatal EVD, and suggest that Ebola virus infection may induce aberrant neutrophils whose activity could explain hallmarks of fatal EVD. Moreover, integrated biomarker prediction identified putative biomarkers from different data platforms that differentiated survivors and fatalities early after infection. This work reveals insight into EVD pathogenesis, suggests an effective approach for biomarker identification, and provides an important community resource for further analysis of human EVD severity.

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