U.S. Department of Energy

Pacific Northwest National Laboratory

The future of liquid chromatography-mass spectrometry (LC-MS) in metabolic profiling and metabolomic studies for biomarker discovery.

TitleThe future of liquid chromatography-mass spectrometry (LC-MS) in metabolic profiling and metabolomic studies for biomarker discovery.
Publication TypeJournal Article
Year of Publication2007
AuthorsMetz TO, Zhang Q, Page JS, Shen Y, Callister SJ, Jacobs JM, Smith RD
JournalBiomark Med
Abstract

The future utility of liquid chromatography-mass spectrometry (LC-MS) in metabolic profiling and metabolomic studies for biomarker discover will be discussed, beginning with a brief description of the evolution of metabolomics and the utilization of the three most popular analytical platforms in such studies: NMR, GC-MS, and LC-MS. Emphasis is placed on recent developments in high-efficiency LC separations, sensitive electrospray ionization approaches, and the benefits to incorporating both in LC-MS-based approaches. The advantages and disadvantages of various quantitative approaches are reviewed, followed by the current LC-MS-based tools available for candidate biomarker characterization and identification. Finally, a brief prediction on the future path of LC-MS-based methods in metabolic profiling and metabolomic studies is given.

DOI10.2217/17520363.1.1.159
Alternate JournalBiomark Med
PubMed ID19177179
PubMed Central IDPMC2631423
Grant ListP41 RR018522-04 / RR / NCRR NIH HHS / United States
R21 DK071283-02 / DK / NIDDK NIH HHS / United States
R33 DK070146-03 / DK / NIDDK NIH HHS / United States
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