U.S. Department of Energy

Pacific Northwest National Laboratory

Advances in microscale separations towards nanoproteomics applications

TitleAdvances in microscale separations towards nanoproteomics applications
Publication TypeJournal Article
Year of Publication2017
AuthorsYi L., Piehowski P.D, Shi T., Smith R.D, Qian W.J
JournalJ Chromatogr A
Abstract

Microscale separation (e.g., liquid chromatography or capillary electrophoresis) coupled with mass spectrometry (MS) has become the primary tool for advanced proteomics, an indispensable technology for gaining understanding of complex biological processes. In recent decades significant advances have been achieved in MS-based proteomics. However, the current proteomics platforms still face an analytical challenge in overall sensitivity towards nanoproteomics applications for starting materials of less than 1μg total proteins (e.g., cellular heterogeneity in tissue pathologies). Herein, we review recent advances in microscale separation techniques and integrated sample processing strategies that improve the overall sensitivity and proteome coverage of the proteomics workflow, and their contributions towards nanoproteomics applications.

| Pacific Northwest National Laboratory