U.S. Department of Energy

Pacific Northwest National Laboratory

Activity-based protein profiling of microbes.

TitleActivity-based protein profiling of microbes.
Publication TypeJournal Article
Year of Publication2015
AuthorsSadler NC, Wright AT
JournalCurr Opin Chem Biol
Abstract

Activity-based protein profiling (ABPP) in conjunction with multimodal characterization techniques has yielded impactful findings in microbiology, particularly in pathogen, bioenergy, drug discovery, and environmental research. Using small molecule chemical probes that react irreversibly with specific proteins or protein families in complex systems has provided insights in enzyme functions in central metabolic pathways, drug-protein interactions, and regulatory protein redox, for systems ranging from photoautotrophic cyanobacteria to mycobacteria, and combining live cell or cell extract ABPP with proteomics, molecular biology, modeling, and other techniques has greatly expanded our understanding of these systems. New opportunities for application of ABPP to microbial systems can enhance protein annotation, characterize protein activities in myriad environments, and reveal signal transduction and regulatory mechanisms in microbial systems.

DOI10.1016/j.cbpa.2014.10.022
Alternate JournalCurr Opin Chem Biol
PubMed ID25531039
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