U.S. Department of Energy

Pacific Northwest National Laboratory

Proteomic profiling of nonenzymatically glycated proteins in human plasma and erythrocyte membranes.

TitleProteomic profiling of nonenzymatically glycated proteins in human plasma and erythrocyte membranes.
Publication TypeJournal Article
Year of Publication2008
AuthorsZhang Q, Tang N, Schepmoes AA, Phillips LS, Smith RD, Metz TO
JournalJ Proteome Res
KeywordsAmino Acid Sequence, Blood Proteins, Diabetes Mellitus, Type 2, Erythrocyte Membrane, Gene Expression Profiling, Glycosylation, Humans, Molecular Sequence Data, Peptides, Plasma, Proteome, Random Allocation
Abstract

Nonenzymatic glycation of peptides and proteins by d-glucose has important implications in the pathogenesis of diabetes mellitus, particularly in the development of diabetic complications. In this work, we report the first proteomics-based characterization of nonenzymatically glycated proteins in human plasma and erythrocyte membranes from individuals with normal glucose tolerance, impaired glucose tolerance, and type 2 diabetes mellitus. Phenylboronate affinity chromatography was used to enrich glycated proteins and glycated tryptic peptides from both human plasma and erythrocyte membranes. The enriched peptides were subsequently analyzed by liquid chromatography coupled with electron transfer dissociation-tandem mass spectrometry, resulting in the confident identification of 76 and 31 proteins from human plasma and erythrocyte membranes, respectively. Although most of the glycated proteins could be identified in samples from individuals with normal glucose tolerance, slightly higher numbers of glycated proteins and more glycation sites were identified in samples from individuals with impaired glucose tolerance and type 2 diabetes mellitus.

DOI10.1021/pr700763r
Alternate JournalJ. Proteome Res.
PubMed ID18396901
PubMed Central IDPMC2731429
Grant ListDK066204 / DK / NIDDK NIH HHS / United States
DK071283 / DK / NIDDK NIH HHS / United States
P41 RR018522-06 / RR / NCRR NIH HHS / United States
R33 DK071283-03 / DK / NIDDK NIH HHS / United States
RR00039 / RR / NCRR NIH HHS / United States
RR018522 / RR / NCRR NIH HHS / United States
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