U.S. Department of Energy

Pacific Northwest National Laboratory

Proteome analysis of Borrelia burgdorferi response to environmental change.

TitleProteome analysis of Borrelia burgdorferi response to environmental change.
Publication TypeJournal Article
Year of Publication2010
AuthorsAngel TE, Luft BJ, Yang X, Nicora CD, Camp DG, Jacobs JM, Smith RD
JournalPLoS One
KeywordsBacterial Proteins, Borrelia burgdorferi, Cluster Analysis, Hydrogen-Ion Concentration, Proteome, Proteomics, Temperature
Abstract

We examined global changes in protein expression in the B31 strain of Borrelia burgdorferi, in response to two environmental cues (pH and temperature) chosen for their reported similarity to those encountered at different stages of the organism's life cycle. Multidimensional nano-liquid chromatographic separations coupled with tandem mass spectrometry were used to examine the array of proteins (i.e., the proteome) of B. burgdorferi for different pH and temperature culture conditions. Changes in pH and temperature elicited in vitro adaptations of this spirochete known to cause Lyme disease and led to alterations in protein expression that are associated with increased microbial pathogenesis. We identified 1,031 proteins that represent 59% of the annotated genome of B. burgdorferi and elucidated a core proteome of 414 proteins that were present in all environmental conditions investigated. Observed changes in protein abundances indicated varied replicon usage, as well as proteome functional distributions between the in vitro cell culture conditions. Surprisingly, the pH and temperature conditions that mimicked B. burgdorferi residing in the gut of a fed tick showed a marked reduction in protein diversity. Additionally, the results provide us with leading candidates for exploring how B. burgdorferi adapts to and is able to survive in a wide variety of environmental conditions and lay a foundation for planned in situ studies of B. burgdorferi isolated from the tick midgut and infected animals.

DOI10.1371/journal.pone.0013800
Alternate JournalPLoS ONE
PubMed ID21072190
PubMed Central IDPMC2970547
Grant ListP41 RR018522-08 / RR / NCRR NIH HHS / United States
RR18522 / RR / NCRR NIH HHS / United States
U01-AI56480 / AI / NIAID NIH HHS / United States
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