Perturbations in the lipid profile of individuals with newly diagnosed type 1 diabetes mellitus: lipidomics analysis of a Diabetes Antibody Standardization Program sample subset.
| Title | Perturbations in the lipid profile of individuals with newly diagnosed type 1 diabetes mellitus: lipidomics analysis of a Diabetes Antibody Standardization Program sample subset. |
| Publication Type | Journal Article |
| Year of Publication | 2010 |
| Authors | Sorensen CM, Ding J, Zhang Q, Alquier T, Zhao R, Mueller PW, Smith RD, Metz TO |
| Journal | Clin Biochem |
| Keywords | Adolescent, Adult, Child, Chromatography, Liquid, Diabetes Mellitus, Type 1, Female, Humans, Lipids, Male, Young Adult |
| Abstract | OBJECTIVES: To characterize the lipid profile of individuals with newly diagnosed type 1 diabetes mellitus using LC-MS-based lipidomics and the accurate mass and time (AMT) tag approach. DESIGN AND METHODS: Lipids were extracted from plasma and sera of 10 subjects from the Diabetes Antibody Standardization Program (years 2000-2005) and 10 non-diabetic subjects and analyzed by capillary liquid chromatography coupled with a hybrid ion-trap-Fourier transform ion cyclotron resonance mass spectrometer. Lipids were identified and quantified using the AMT tag approach. RESULTS: Five hundred fifty-nine lipid features differentiated (q<0.05) diabetic from healthy individuals in a partial least-squares analysis, characterizing individuals with recently diagnosed type 1 diabetes mellitus. CONCLUSIONS: A lipid profile associated with newly diagnosed type 1 diabetes may aid in further characterization of biochemical pathways involved in lipid regulation or mobilization. |
| DOI | 10.1016/j.clinbiochem.2010.04.075 |
| Alternate Journal | Clin. Biochem. |
| PubMed ID | 20519132 |
| PubMed Central ID | PMC2919299 |
| Grant List | DK070146 / DK / NIDDK NIH HHS / United States R33 DK070146-04 / DK / NIDDK NIH HHS / United States |