U.S. Department of Energy

Pacific Northwest National Laboratory

Perturbations in the lipid profile of individuals with newly diagnosed type 1 diabetes mellitus: lipidomics analysis of a Diabetes Antibody Standardization Program sample subset.

TitlePerturbations in the lipid profile of individuals with newly diagnosed type 1 diabetes mellitus: lipidomics analysis of a Diabetes Antibody Standardization Program sample subset.
Publication TypeJournal Article
Year of Publication2010
AuthorsSorensen CM, Ding J, Zhang Q, Alquier T, Zhao R, Mueller PW, Smith RD, Metz TO
JournalClin Biochem
KeywordsAdolescent, Adult, Child, Chromatography, Liquid, Diabetes Mellitus, Type 1, Female, Humans, Lipids, Male, Young Adult
Abstract

OBJECTIVES: To characterize the lipid profile of individuals with newly diagnosed type 1 diabetes mellitus using LC-MS-based lipidomics and the accurate mass and time (AMT) tag approach.


DESIGN AND METHODS: Lipids were extracted from plasma and sera of 10 subjects from the Diabetes Antibody Standardization Program (years 2000-2005) and 10 non-diabetic subjects and analyzed by capillary liquid chromatography coupled with a hybrid ion-trap-Fourier transform ion cyclotron resonance mass spectrometer. Lipids were identified and quantified using the AMT tag approach.


RESULTS: Five hundred fifty-nine lipid features differentiated (q<0.05) diabetic from healthy individuals in a partial least-squares analysis, characterizing individuals with recently diagnosed type 1 diabetes mellitus.


CONCLUSIONS: A lipid profile associated with newly diagnosed type 1 diabetes may aid in further characterization of biochemical pathways involved in lipid regulation or mobilization.

DOI10.1016/j.clinbiochem.2010.04.075
Alternate JournalClin. Biochem.
PubMed ID20519132
PubMed Central IDPMC2919299
Grant ListDK070146 / DK / NIDDK NIH HHS / United States
R33 DK070146-04 / DK / NIDDK NIH HHS / United States
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