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A perspective on the Maillard reaction and the analysis of protein glycation by mass spectrometry: probing the pathogenesis of chronic disease.

TitleA perspective on the Maillard reaction and the analysis of protein glycation by mass spectrometry: probing the pathogenesis of chronic disease.
Publication TypeJournal Article
Year of Publication2009
AuthorsZhang Q, Ames JM, Smith RD, Baynes JW, Metz TO
JournalJ Proteome Res
KeywordsAnimals, Carbohydrate Conformation, Carbohydrate Sequence, Chronic Disease, Glycosylation End Products, Advanced, Humans, Maillard Reaction, Mass Spectrometry, Molecular Structure, Proteins, Proteomics
Abstract

The Maillard reaction, starting from the glycation of protein and progressing to the formation of advanced glycation end-products (AGEs), is implicated in the development of complications of diabetes mellitus, as well as in the pathogenesis of cardiovascular, renal, and neurodegenerative diseases. In this perspective review, we provide an overview on the relevance of the Maillard reaction in the pathogenesis of chronic disease and discuss traditional approaches and recent developments in the analysis of glycated proteins by mass spectrometry. We propose that proteomics approaches, particularly bottom-up proteomics, will play a significant role in analyses of clinical samples leading to the identification of new markers of disease development and progression.

DOI10.1021/pr800858h
Alternate JournalJ. Proteome Res.
PubMed ID19093874
PubMed Central IDPMC2642649
Grant ListDK071283 / DK / NIDDK NIH HHS / United States
DK19971 / DK / NIDDK NIH HHS / United States
R33 DK071283-04 / DK / NIDDK NIH HHS / United States
R37 DK019971-32 / DK / NIDDK NIH HHS / United States
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