Metabolomic response of human skin tissue to low dose ionizing radiation.
Title | Metabolomic response of human skin tissue to low dose ionizing radiation. |
Publication Type | Journal Article |
Year of Publication | 2012 |
Authors | Hu Z-P, Kim Y-M, Sowa MB, R Robinson J, Gao X, Metz TO, Morgan WF, Zhang Q |
Journal | Mol Biosyst |
Keywords | Biological Markers, Cell Line, Cells, Cultured, DNA Damage, DNA Repair, Dose-Response Relationship, Radiation, Humans, Metabolomics, Nucleic Acids, Radiation Dosage, Radiation Protection, Skin, Systems Biology |
Abstract | Understanding how human organs respond to ionizing radiation (IR) at a systems biology level and identifying biomarkers for IR exposure at low doses can help provide a scientific basis for establishing radiation protection standards. Little is known regarding the physiological responses to low dose IR at the metabolite level, which represents the end-point of biochemical processes inside cells. Using a full thickness human skin tissue model and GC-MS-based metabolomic analysis, we examined the metabolic perturbations at three time points (3, 24 and 48 h) after exposure to 3, 10 and 200 cGy of X-rays. PLS-DA score plots revealed dose- and time-dependent clustering between sham and irradiated groups. Importantly, delayed metabolic responses were observed at low dose IR. When compared with the high dose at 200 cGy, a comparable number of significantly changed metabolites were detected 48 h after exposure to low doses (3 and 10 cGy) of irradiation. Biochemical pathway analysis showed perturbations to DNA/RNA damage and repair, lipid and energy metabolisms, even at low doses of IR. |
DOI | 10.1039/c2mb25061f |
Alternate Journal | Mol Biosyst |
PubMed ID | 22610363 |