U.S. Department of Energy

Pacific Northwest National Laboratory

Increasing confidence of LC–MS identifications by utilizing ion mobility spectrometry

TitleIncreasing confidence of LC–MS identifications by utilizing ion mobility spectrometry
Publication TypeJournal Article
Year of Publication2013
AuthorsCrowell KL, Baker ES, Payne SH, Ibrahim YM, Monroe ME, Slysz GW, LaMarche BL, Petyuk VA, Piehowski PD, Danielson, III WF, Anderson GA, Smith RD
JournalInternational Journal of Mass Spectrometry

Ion mobility spectrometry in conjunction with liquid chromatography separations and mass spectrometry offers a range of new possibilities for analyzing complex biological samples. To fully utilize the information obtained from these three measurement dimensions, informatics tools based on the accurate mass and time tag methodology were modified to incorporate ion mobility spectrometry drift times for peptides observed in human serum. In this work a reference human serum database was created for 12,139 peptides and populated with the monoisotopic mass, liquid chromatography normalized elution time, and ion mobility spectrometry drift time(s) for each. We demonstrate that the use of three dimensions for peak matching during the peptide identification process resulted in an increased numbers of identifications and a lower false discovery rate relative to only using the mass and normalized elution time dimensions.

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