U.S. Department of Energy

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Implementation of dipolar resonant excitation for collision induced dissociation with ion mobility/time-of-flight MS.

TitleImplementation of dipolar resonant excitation for collision induced dissociation with ion mobility/time-of-flight MS.
Publication TypeJournal Article
Year of Publication2014
AuthorsWebb IK, Chen T-C, Danielson WF, Ibrahim YM, Tang K, Anderson GA, Smith RD
JournalJ Am Soc Mass Spectrom
Abstract

An ion mobility/time-of-flight mass spectrometer (IMS/TOF MS) platform that allows for resonant excitation collision induced dissociation (CID) is presented. Highly efficient, mass-resolved fragmentation without additional excitation of product ions was accomplished and over-fragmentation common in beam-type CID experiments was alleviated. A quadrupole ion guide was modified to apply a dipolar AC signal across a pair of rods for resonant excitation. The method was characterized with singly protonated methionine enkephalin and triply protonated peptide angiotensin I, yielding maximum CID efficiencies of 44% and 84%, respectively. The Mathieu q(x,y) parameter was set at 0.707 for these experiments to maximize pseudopotential well depths and CID efficiencies. Resonant excitation CID was compared with beam-type CID for the peptide mixture. The ability to apply resonant waveforms in mobility-resolved windows is demonstrated with a peptide mixture yielding fragmentation over a range of mass-to-charge (m/z) ratios within a single IMS-MS analysis.

DOI10.1007/s13361-013-0815-6
Alternate JournalJ. Am. Soc. Mass Spectrom.
PubMed ID24470195
Grant ListGM103493 / GM / NIGMS NIH HHS / United States
P41 GM103493 / GM / NIGMS NIH HHS / United States
Area of Research: 
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