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High Resolution Separations and Improved Ion Production and Transmission in Metabolomics.

TitleHigh Resolution Separations and Improved Ion Production and Transmission in Metabolomics.
Publication TypeJournal Article
Year of Publication2008
AuthorsMetz TO, Page JS, Baker ES, Tang K, Ding J, Shen Y, Smith RD
JournalTrends Analyt Chem
Abstract

The goal of metabolomics analyses is the detection and quantitation of as many sample components as reasonably possible in order to identify compounds or "features" that can be used to characterize the samples under study. When utilizing electrospray ionization to produce ions for analysis by mass spectrometry (MS), it is important that metabolome sample constituents be efficiently separated prior to ion production, in order to minimize ionization suppression and thereby extend the dynamic range of the measurement, as well as the coverage of the metabolome. Similarly, optimization of the MS inlet and interface can lead to increased measurement sensitivity. This perspective review will focus on the role of high resolution liquid chromatography (LC) separations in conjunction with improved ion production and transmission for LC-MS-based metabolomics. Additional emphasis will be placed on the compromise between metabolome coverage and sample analysis throughput.

DOI10.1016/j.trac.2007.11.003
Alternate JournalTrends Analyt Chem
PubMed ID19255623
PubMed Central IDPMC2625305
Grant ListP41 RR018522-05 / RR / NCRR NIH HHS / United States
R21 CA126191-01 / CA / NCI NIH HHS / United States
R33 DK071283-03 / DK / NIDDK NIH HHS / United States
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