Experimental evaluation and optimization of structures for lossless ion manipulations for ion mobility spectrometry with time-of-flight mass spectrometry.
Title | Experimental evaluation and optimization of structures for lossless ion manipulations for ion mobility spectrometry with time-of-flight mass spectrometry. |
Publication Type | Journal Article |
Year of Publication | 2014 |
Authors | Webb IK, Garimella SVB, Tolmachev AV, Chen T-C, Zhang X, Norheim RV, Prost SA, LaMarche B, Anderson GA, Ibrahim YM, Smith RD |
Journal | Anal Chem |
Keywords | Electricity, Electrodes, Ions, Radio Waves, Spectrometry, Mass, Electrospray Ionization |
Abstract | We report on the performance of structures for lossless ion manipulation (SLIM) as a means for transmitting ions and performing ion mobility separations (IMS). Ions were successfully transferred from an electrospray ionization (ESI) source to the TOF MS analyzer by means of a linear SLIM, demonstrating lossless ion transmission and an alternative arrangement including a 90° turn. First, the linear geometry was optimized for radial confinement by tuning RF on the central "rung" electrodes and potentials on the DC-only guard electrodes. Selecting an appropriate DC guard bias (2-6 V) and RF amplitude (≥160 V(p-p) at 750 kHz) resulted in the greatest ion intensities. Close to ideal IMS resolving power was maintained over a significant range of applied voltages. Second, the 90° turn was optimized for radial confinement by tuning RF on the rung electrodes and DC on the guard electrodes. However, both resolving power and ion transmission showed a dependence on these voltages, and the best conditions for both were >300 V(p-p) RF (685 kHz) and 7-11 V guard DC bias. Both geometries provide IMS resolving powers at the theoretical limit (R ~ 58), showing that degraded resolution from a "racetrack" effect from turning around a corner can be successfully avoided, and the capability also was maintained for essentially lossless ion transmission. |
DOI | 10.1021/ac502055e |
Alternate Journal | Anal. Chem. |
PubMed ID | 25152066 |
PubMed Central ID | PMC4165449 |
Grant List | P41 GM103493 / GM / NIGMS NIH HHS / United States P41 GM103493 / GM / NIGMS NIH HHS / United States R21 GM103497 / GM / NIGMS NIH HHS / United States R21 GM103497 / GM / NIGMS NIH HHS / United States |