U.S. Department of Energy

Pacific Northwest National Laboratory

Development of a fibrinogen-specific sandwich enzyme-linked immunosorbent assay microarray assay for distinguishing between blood plasma and serum samples.

TitleDevelopment of a fibrinogen-specific sandwich enzyme-linked immunosorbent assay microarray assay for distinguishing between blood plasma and serum samples.
Publication TypeJournal Article
Year of Publication2011
AuthorsGonzalez RM, Zhang Q, Zangar RC, Smith RD, Metz TO
JournalAnal Biochem
KeywordsAnimals, Antibodies, Antibodies, Monoclonal, Enzyme-Linked Immunosorbent Assay, Equipment Design, Fibrinogen, Humans, Plasma, Protein Array Analysis, Proteomics, Sensitivity and Specificity, Serum
Abstract

We have developed a fibrinogen-specific sandwich enzyme-linked immunosorbent assay (ELISA) microarray assay for use in qualitatively distinguishing between blood plasma and serum samples. Three capture antibodies (49D2, HPA001900, and F8512) were evaluated in conjunction with 1D6 as the detection antibody. The data show that 49D2 and (to a lesser extent) F8512 successfully identify previously unknown plasma and serum samples based on approximately a 28-fold difference in signal intensity between the sample types. This assay has utility in rapidly identifying previously archived clinical samples with incomplete annotation in a high-throughput manner prior to proteomic analyses.

DOI10.1016/j.ab.2011.02.039
Alternate JournalAnal. Biochem.
PubMed ID21371419
PubMed Central IDPMC3097095
Grant ListDK070146 / DK / NIDDK NIH HHS / United States
EB006177 / EB / NIBIB NIH HHS / United States
R01 EB006177-03 / EB / NIBIB NIH HHS / United States
R33 DK070146-04 / DK / NIDDK NIH HHS / United States
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