U.S. Department of Energy

Pacific Northwest National Laboratory

Comprehensive identification of glycated peptides and their glycation motifs in plasma and erythrocytes of control and diabetic subjects.

TitleComprehensive identification of glycated peptides and their glycation motifs in plasma and erythrocytes of control and diabetic subjects.
Publication TypeJournal Article
Year of Publication2011
AuthorsZhang Q, Monroe ME, Schepmoes AA, Clauss TRW, Gritsenko MA, Meng D, Petyuk VA, Smith RD, Metz TO
JournalJ Proteome Res
KeywordsAmino Acid Motifs, Biological Markers, Blood Proteins, Chromatography, Affinity, Chromatography, High Pressure Liquid, Diabetes Complications, Diabetes Mellitus, Type 2, Erythrocytes, Glycopeptides, Glycosylation, Glycosylation End Products, Advanced, Humans, Hyperglycemia, Molecular Sequence Data, Peptide Fragments, Plasma, Proteomics, Tandem Mass Spectrometry, Trypsin
Abstract

Nonenzymatic glycation of proteins sets the stage for formation of advanced glycation end-products and development of chronic complications of diabetes. In this report, we extended our previous methods on proteomics analysis of glycated proteins to comprehensively identify glycated proteins in control and diabetic human plasma and erythrocytes. Using immunodepletion, enrichment, and fractionation strategies, we identified 7749 unique glycated peptides, corresponding to 3742 unique glycated proteins. Semiquantitative comparisons showed that glycation levels of a number of proteins were significantly increased in diabetes and that erythrocyte proteins were more extensively glycated than plasma proteins. A glycation motif analysis revealed that some amino acids were favored more than others in the protein primary structures in the vicinity of the glycation sites in both sample types. The glycated peptides and corresponding proteins reported here provide a foundation for potential identification of novel markers for diabetes, hyperglycemia, and diabetic complications in future studies.

DOI10.1021/pr200040j
PubMed ID21612289
PubMed Central IDPMC3128674
Grant ListDK071283 / DK / NIDDK NIH HHS / United States
P41 RR018522-08 / RR / NCRR NIH HHS / United States
R33 DK071283-05 / DK / NIDDK NIH HHS / United States
RR018522 / RR / NCRR NIH HHS / United States
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