U.S. Department of Energy

Pacific Northwest National Laboratory

Application of the accurate mass and time tag approach in studies of the human blood lipidome.

TitleApplication of the accurate mass and time tag approach in studies of the human blood lipidome.
Publication TypeJournal Article
Year of Publication2008
AuthorsDing J, Sorensen CM, Jaitly N, Jiang H, Orton DJ, Monroe ME, Moore RJ, Smith RD, Metz TO
JournalJ Chromatogr B Analyt Technol Biomed Life Sci
KeywordsChromatography, Liquid, Erythrocytes, Humans, Lipids, Lymphocytes, Tandem Mass Spectrometry
Abstract

We report a preliminary demonstration of the accurate mass and time (AMT) tag approach for lipidomics. Initial data-dependent LC-MS/MS analyses of human plasma, erythrocyte, and lymphocyte lipids were performed in order to identify lipid molecular species in conjunction with complementary accurate mass and isotopic distribution information. Identified lipids were used to populate initial lipid AMT tag databases containing 250 and 45 entries for those species detected in positive and negative electrospray ionization (ESI) modes, respectively. The positive ESI database was then utilized to identify human plasma, erythrocyte, and lymphocyte lipids in high-throughput LC-MS analyses based on the AMT tag approach. We were able to define the lipid profiles of human plasma, erythrocytes, and lymphocytes based on qualitative and quantitative differences in lipid abundance.

DOI10.1016/j.jchromb.2008.04.040
Alternate JournalJ. Chromatogr. B Analyt. Technol. Biomed. Life Sci.
PubMed ID18502191
PubMed Central IDPMC2598838
Grant ListDK070146 / DK / NIDDK NIH HHS / United States
DK071283 / DK / NIDDK NIH HHS / United States
R33 DK070146-04 / DK / NIDDK NIH HHS / United States
R33 DK071283-03 / DK / NIDDK NIH HHS / United States
| Pacific Northwest National Laboratory