SpectrumLook
SpectrumLook can be used to inspect the fragmentation (MS/MS) spectra in an LC-MS/MS analysis. It reads Finnigan .Raw files, .mzXml files, or .mzData files, plus a "Synopsis File" listing peptides identified by a MS/MS interpretation tool like SEQUEST or X!Tandem. The software allows users to visually browse the MS/MS spectra that led to the peptide identifications, including viewing annotations for the identified b and y ions, plus neutral loss ions where appropriate.
Note that you can generate SEQUEST Synopsis/First Hits files from SEQUEST .Out files using the Peptide File Extractor.
| Download Software Tool | Download Source Code |
| Version | v1.5.37 | Requirements | Microsoft Windows (Program installs VB6 runtime if necessary) |
| Date Updated | October 16, 2009 | File Size (Software Tool) | 5.5 MB (ZIP) |
| Registration Required | No | File size (Source Code) | 4.4 MB (ZIP) |
| Developers | Matthew Monroe | ||
| Comments | See the complete Revision History for a history of changes | ||
Acknowledgment
All publications that utilize this software should provide appropriate acknowledgement to PNNL and the OMICS.PNL.GOV website. However, if the software is extended or modified, then any subsequent publications should include a more extensive statement, using this text or a similar variant:
Portions of this research were supported by the NIH National Center for Research Resources (Grant RR018522), the W.R. Wiley Environmental Molecular Science Laboratory (a national scientific user facility sponsored by the U.S. Department of Energy's Office of Biological and Environmental Research and located at PNNL), and the National Institute of Allergy and Infectious Diseases (NIH/DHHS through interagency agreement Y1-AI-4894-01). PNNL is operated by Battelle Memorial Institute for the U.S. Department of Energy under contract DE-AC05-76RL0 1830.