MultiAlign
The MultiAlign program can read ICR2LS and Decon2LS files and match the features found to an LC-MS/MS Accurate Mass and Time Tag database generated by the MTDBCreator application. If using MultiAlign inside PNNL, you can also load data from the Mass Tag System (MTS).
The MultiAlign application provides advanced visualization and manipulation capabilities for LC-MS datasets acquired on high resolution mass spectrometers. Functionalities include overlaid 2D plots, alignment plots, normalizations, and basic statistical comparisons. MultiAlign uses the LCMSWARP algorithm to consolidate features from multiple LC-MS datasets into a master list, then aligns the data to an AMT tag database to identify the LC-MS features. Data processing is similar to that performed with the VIPER application, but MultiAlign has the advantage of first aligning multiple datasets to one another, followed by alignment and matching to an AMT tag DB. The processing performed by MultiAlign results in fewer "missing values" vs. the results obtained when analyzing separate datasets with VIPER.
After installing, please see the Readme.txt file for additional details on using MultiAlign. Note that several example data files will be included in the MultiAlign program folder
| Download Software Tool |
| Version | v1.1.2994 | Requirements | Microsoft NET Framework 1.1 |
| Date Updated | March 13, 2008 | File Size (Software Tool) | 25 MB (ZIP) |
| Registration Required | No | File size (Source Code) | n/a |
| Developers | Navdeep Jaitly, Brian LaMarche, Ashoka Polpitya, Derek Hopkins | ||
| Comments | See the complete Revision History for a history of changes | ||
Application Details
Cluster Chart
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The main window in MultiAlign shows the alignment of the features of multiple datasets at the top and the feature details at the bottom. After matching to a database, the details on the matching peptides are also displayed at the bottom. |
Dataset Alignment Window
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To align multiple datasets, you first choose a baseline dataset, and then the program aligns the data to the baseline dataset. The alignment window allows you to view the alignment function used to align each dataset to the baseline dataset. |
Acknowledgment
All publications that utilize this software should provide appropriate acknowledgement to PNNL and the OMICS.PNL.GOV website. However, if the software is extended or modified, then any subsequent publications should include a more extensive statement, using this text or a similar variant:
Portions of this research were supported by the NIH National Center for Research Resources (Grant RR018522), the W.R. Wiley Environmental Molecular Science Laboratory (a national scientific user facility sponsored by the U.S. Department of Energy's Office of Biological and Environmental Research and located at PNNL), and the National Institute of Allergy and Infectious Diseases (NIH/DHHS through interagency agreement Y1-AI-4894-01). PNNL is operated by Battelle Memorial Institute for the U.S. Department of Energy under contract DE-AC05-76RL0 1830.